activin a (Miltenyi Biotec)

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Miltenyi Biotec activin a
Activin A, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/activin+a/Human+Activin+A%2C+premium+grade/pm42249199-45-21-25
Average 94 stars, based on 31 article reviews

activin a - by Bioz Stars, 2026-10

94/100 stars

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Article Title: Ubiquitous MEIS transcription factors actuate lineage-specific transcription to establish cell fate
Article Snippet: Activin A , Miltenyi Biotech , 130-115-009.

Recombinant:

Article Title: EGFR-mediated local invasiveness and response to Cetuximab in head and neck cancer.
Article Snippet: FaDu, Kyse30, Cal27, Cal33, OSC19, HSC4 (ATCC, Manassas, VA, USA and JCRB cell bank, Neuss, Germany) were regularly confirmed via short tandemrepeat typing (STR), mycoplasma tested, and passaged in DMEM, DMEM/HAM F12 (OSC19) or RPMI, 10% FCS, 1% penicillin/streptomycin, 5% CO2 atmosphere at 37 °C. .. Treatment with EGF (50 ng/mL, Gibco Fisher Scientific, Munich, Germany), AREG (1,000 ng/mL) and EREG (80 ng/mL) (Peprotech, PeproTech, Hamburg, Germany), Activin A (Miltenyi Biotec, 130–115-012), Cetuximab (10 μg/mL, Erbitux, Merck Serono, Darmstadt, Germany), MEK1/2 (MAPK–ERK kinase) inhibitor AZD6244 (100 nM, Selleckchem, Munich, Germany), Akt signaling pathway inhibitor MK2206 (1 μM; Selleckchem, Munich, Germany), SPHK1 inhibitor PF-543 (5–10 μM, Selleckchem, Munich, Germany, s7177), Follistatin (20 ng/mL, PeproTech, Hamburg, Germany), recombinant human laminin 332 (10 μg/ml for coating and 1.6–9.0 nM for treatment and complementation of type I collagen, Biolaminin 332 LN, BioLaminaAB, Sundbyberg, Sweden), sphingosine-1-phosphate (1.5 mM, MedChemExpress, South Brunswick, US) were conducted under serum-free conditions. .. Cells were stained with ITGB4-specific antibody (439- 9B, Thermo FisherScientific, Hamburg, Germany),1:100 dilution in PBS-3% FCS, 60 min on ice, washed three times in PBS-3% FCS, and stained with FITC-conjugated antibody (1:100, 40 min at 4 °C, FI-4001, Vector Laboratories/Biozol, Eching, Germany).

Activation Assay:

Article Title: Deciphering the Origin and Plasticity of Circulating Endothelial Cells: A Model for Systemic Angiogenesis Programs in Health and Disease
Article Snippet: .. Two differential conditions were applied: (1) a non-direct Notch activation, using CultEC growth medium excluding the kit-supplied VEGF, supplemented with only 2% FBS, vascular endothelial growth factor (VEGF; 100 ng/mL, Cat. no. 130-109-383, Miltenyi Biotec), and Activin A (25 ng/mL, Cat. no. 130-115-008, Miltenyi Biotec)., (2) A direct Notch inhibition, using CultEC growth medium supplemented with 10% FBS and the γ-secretase inhibitor RO4929097 (1 μM, Cat. no. sc-364602, Santa Cruz Biotechnology). ..

Inhibition:

Cell Culture:

Article Title: Animal component-free differentiation of human pluripotent stem cells into macrophages.
Article Snippet: .. Briefly, from day 0 to day 2, cells were cultured in IF9S medium supplemented with 15 ng/mL Activin A (Miltenyi Biotec, 130–115-010), 40 ng/mL BMP-4 (R&D Systems, 314-BP-050), and 1.5 μM CHIR99021 (Tocris, 4423). .. From day 2 to day 5, the medium was supplemented with 50 ng/mL VEGF-A (Miltenyi Biotec, 130–109-386), 50 ng/mL bFGF (Miltenyi Biotec, 130–093-842), 50 ng/mL SCF (Miltenyi Biotec, 130–096-695), and 10 μM SB431542 (Tocris Bioscience, 1614).

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